Review



nuclear dye 4  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher nuclear dye 4
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Nuclear Dye 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/pmc13196778-213-20-23
    Average 99 stars, based on 1 article reviews
    nuclear dye 4 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases"

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    Journal: Science Advances

    doi: 10.1126/sciadv.aed1296

    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.
    Figure Legend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Techniques Used: Staining, Microarray



    Similar Products

    dapi dye  (Beyotime)
    99
    Beyotime dapi dye
    Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pmc13123509-556-26-32
    Average 99 stars, based on 1 article reviews
    dapi dye - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    dyes 4  (MedChemExpress)
    98
    MedChemExpress dyes 4
    Dyes 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pm42430828-62-2-14
    Average 98 stars, based on 1 article reviews
    dyes 4 - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier
    nuclear dye 4  (Thermo Fisher)
    99
    Thermo Fisher nuclear dye 4
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Nuclear Dye 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/pmc13196778-213-20-23
    Average 99 stars, based on 1 article reviews
    nuclear dye 4 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    dapi nuclear dye  (Fisher Scientific)
    86
    Fisher Scientific dapi nuclear dye
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Dapi Nuclear Dye, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/dapi+dye+nuclear/pm42103073-172-6-11
    Average 86 stars, based on 1 article reviews
    dapi nuclear dye - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    dapi dye  (Servicebio Inc)
    86
    Servicebio Inc dapi dye
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Dapi Dye, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/dapi/pm42058628-589-19-4
    Average 86 stars, based on 1 article reviews
    dapi dye - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    dapi dye solution  (Beyotime)
    99
    Beyotime dapi dye solution
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Dapi Dye Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pmc13130657-60-0-4
    Average 99 stars, based on 1 article reviews
    dapi dye solution - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    dye 4  (Thermo Fisher)
    99
    Thermo Fisher dye 4
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Dye 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/us12600800-746-6-10
    Average 99 stars, based on 1 article reviews
    dye 4 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    diamidino 2 phenylindole dapi dye  (Beyotime)
    99
    Beyotime diamidino 2 phenylindole dapi dye
    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
    Diamidino 2 Phenylindole Dapi Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye+dapi/DAPI/pm41950674-49-12-17
    Average 99 stars, based on 1 article reviews
    diamidino 2 phenylindole dapi dye - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray